COS 180-1 - Optimal resource investment to photosynthetic capacity controls leaf and whole plant acclimation responses to elevated CO2

Abstract: Plants acclimate to increasing CO₂ by reducing leaf nutrient allocation and photosynthetic capacity at the leaf level, a response that often occurs alongside growth stimulation at the whole plant level. Nutrient limitation has been hypothesized to be the primary mechanism driving leaf and whole plant acclimation responses to CO₂, as nutrient availability commonly limits primary productivity and may decrease with increasing CO₂ over time. However, recent work leveraging photosynthetic least-cost theory indicates that these acclimation responses may instead be the result of optimal resource investment toward photosynthetic capacity, which maximizes nutrient allocation to whole plant growth. Acclimation responses to CO₂ may also vary in species with different nutrient acquisition strategies, but few studies have examined these responses across a soil nitrogen availability gradient and in species with different nutrient acquisition strategies. To test whether nutrient limitation or optimal leaf resource investment controls leaf and whole plant acclimation responses to CO₂ and how nutrient acquisition strategy modifies these responses, we grew Glycine max L. (Merr) seedlings under two atmospheric CO₂ levels, with and without Bradyrhizobium japonicum inoculation, and across nine soil nitrogen fertilization treatments in a full factorial growth chamber experiment. After seven weeks, G. max demonstrated a strong downregulation in leaf nitrogen content, V_cmax25, and J_max25 under elevated CO₂, patterns that was not causally linked to changes in soil nitrogen fertilization or inoculation treatment. A relatively stronger downregulation in leaf nitrogen content than V_cmax25 increased the proportion of leaf nitrogen content allocated to photosynthesis, while a relatively stronger downregulation in V_cmax25 than J_max25 stimulated J_max25:V_cmax25 under elevated CO₂. These leaf acclimation responses to elevated CO₂ corresponded with strong stimulations in total leaf area and total biomass, a pattern that was generally enhanced with increasing fertilization and in inoculated pots. Whole plant acclimation responses to CO₂ were driven by reductions in the cost of acquiring nitrogen with increasing fertilization and inoculation. Overall, these results provide strong support for patterns expected from photosynthetic least-cost theory, showing that optimal resource investment is the primary mechanism governing G. max acclimation responses to elevated CO₂.